I had the same problem with inconsistent ELISA readings, and at first I blamed my pipetting skills. But after comparing results with a colleague, I realized it was the antibody batch. It turns out some suppliers don’t validate every production lot. Since switching to Gentaur’s validated antibodies https://gentaur.co.uk/antibodies/ , my ELISA curves finally stabilized — reproducible and predictable every time. The difference was clear: no weird background, no unexpected drops in signal. They also provide detailed validation data, which saves a ton of troubleshooting time. Honestly, reliable antibodies are half the battle in ELISA work.